Background:

Physics (University of Tübingen, Germany, Brown University, RI, USA), Masters at the Max-Planck-Institute for Biology in Tübingen, PhD in Biochemistry (University of Oxford), Research Assistant in Structural Biology (University of Oxford), College Lecturer for Biophysical Chemistry (Wadham College, Oxford), 2003 Lecturer and 2008 Reader for Biological NMR (University of Southampton)

Research interests:

Structural changes of proteins are important for the control of cellular processes and failure to function correctly can lead to disease. We are studying the nature of protein-ligand interactions that have well-characterised biological functions with atomic resolution. The role of protein dynamics in molecular recognition processes is a particular focus of my work.

Please visit BioNMR at Southampton for details on our research.

Interface with Business:

I am a Business Fellow with London Technology Network. On behalf of the School of Biological Sciences I am facilitating academic industrial contacts and liaise with Industry.

Members of the BioNMR Group in 2008:

Experimental Officer in NMR:
Stuart Findlow

Post Docs:
Ludovic Pecqueur
BBSRC : Structural Characterization of a novel HIV Assembly Inhibitor

John Stuart
BBSRC: Order and Dynamics in multidomain protein complexes

Pre Docs:
Clare Cox
(BBSRC studentship)

Michael Knight
(EPSRC studentship)

Garrick Taylor
BBSRC DTA studentship joint with Phil Williamson

Alex Mo
BBSRC joint with Ganesan and Tim Elliott

Visiting Students:
Vanda Bartonova

Previous Members:
Rob Hagan
Stuart Johns
Sina Reckel
Jana Sticht

For vacancies please see Vacancies

Selected publications:

Downing, A. K., Knott, V., Werner, J. M., Cardy, C. M., Campbell, I. D. & Handford, P. A. (1996). Solution structure of a pair of calcium-binding epidermal growth factor-like domains: Implications for the Marfan syndrome and other genetic disorders. Cell85, 597-605

Werner, J.M. , Knott, V., Handford, P.A., Campbell, I.D. & Downing, A.K. (2000) Backbone dynamics of a cbEGF domain pair in the presence of calcium. J.Mol.Biol., 296, 1065-1078.

Hashimoto, Y., Smith, S.P., Pickford, A.R., Bocquier, A.A., Campbell, I.D. & Werner, J.M. (2000) The relative orientation of the fibronectin 6F1 1F2 module pair: A 15N NMR relaxation study. J.Biomol.NMR., 17, 203-214.

Smith, S.P., Hashimoto, Y., Pickford, A.R., Campbell, I.D. & Werner, J.M. (2000) Interface characterisation of the type II module pair from fibronectin. Biochemistry, 39, 8374-8381.

Ulmer, T.S, J.M. Werner, I.D. Campbell (2002) The Relative SH3-SH2 Domain Orientation in Src Kinases . Struct. Fold. Des., 10, 901-911

Schwarz-Linek, U., Werner, J. M., Pickford, A. R., Gurusiddappa, S., Kim, J. H., Pilka, E. S., Briggs, J. A., Gough, T. S., Hook, M., Campbell, I. D. & Potts, J. R. (2003). Pathogenic bacteria attach to human fibronectin through a tandem beta-zipper. Nature423, 177-81.

Hoellerer, M. K., Noble, M. E., Labesse, G., Campbell, I. D., Werner, J. M. & Arold, S. T. (2003). Molecular recognition of paxillin LD motifs by the focal adhesion targeting domain. Structure11, 1207-17.

Altroff, H., Schlinkert, R., van der Walle, C. F., Bernini, A., Campbell, I. D., Werner, J. M. & Mardon, H. J. (2004). Interdomain tilt angle determines integrin-dependent function of the ninth and tenth FIII domains of human fibronectin. J Biol Chem279, 55995-6003.

Sticht, J., Humbert, M., Findlow, S., Bodem, J., Muller, B., Dietrich, U., Werner, J., and Krausslich, H. G. (2005) Nat Struct Mol Biol12(8), 671-677

Erskine, P. T., Beaven, G. D., Hagan, R., Findlow, I. S., Werner, J. M., Wood, S. P., Vernon, J., Giese, K. P., Fox, G., and Cooper, J. B. (2006) J Mol Biol357, 1536-1547

Rachwal, P. A., Findlow, I. S., Werner, J. M., Brown, T., and Fox, K. R. (2007) NAR35, 4214-4222

Rudino-Pinera, E., Ravelli, R. B., Sheldrick, G. M., Nanao, M. H., Korostelev, V. V., Werner, J. M., Schwarz-Linek, U., Potts, J. R., and Garman, E. F. (2007) J Mol Biol368, 833-844

El-Sagheer AH, Kumar R, Findlow IS, Werner JM, Lane AN and Brown T. (2008) A Very stable cyclic DNA mini-duplex with just two base pairs, Chembiochem .; 9, 50-2

Scheschonka A, Findlow, IS, Schemm1 R, El Far O, Caldwell JH, Crump MP, Holden-Dye K, O’Connor V , Betz H, and Werner JM (2008). Structural determinants of calmodulin binding to the intracellular C-terminal domain of the metabotropic glutamate receptor 7A. J Biol Chem. 283, 5577-88

Reviews and book contributions:

Werner, J.M. , Campbell, I.D., Downing, A.K. (2002) Shape and dynamics of a calcium binding EGF domain pair investigated by 15N-NMR relaxation. Methods Mol. Biol. 296, 1065-1078

Rooney, L. M., Sachchidanand & Werner, J. M. (2004). Characterizing domain interfaces by NMR. Methods Mol. Biol.278, 123-38.